Results: PA/CCMTS-P was prepared and characterized as gel with a phase-transition temperature of 32.9☌. In addition, the ability of PA/CCMTS-P to restore the intestinal mucosal barrier after rectal administration was evaluated by immunohistochemical analysis (IHC). The anti-inflammatory effects of PA/CCMTS-P were studied in lipopolysaccharide-induced RAW264.7 cells and dextran sulfate sodium (DSS)-induced ulcerative colitis mouse models. The cytotoxicity and cellular uptake of CCMTS-P were investigated in lipopolysaccharide (LPS) -induced macrophages by CCK-8 assay. CCMTS and aldehyde poloxamer 407 (P407-CHO) were synthesized and chemically cross-linked by Schiff base reaction to formulate thermosensitive in situ gel, which carried Periplaneta americana extracts (PA/CCMTS-P). Materials and methods: Thermosensitive (poloxamer 407) and adhesive polymers (chondroitin sulfate modified carboxymethyl chitosan, CCMTS) were used to construct the in situ gel. Objective: The aim of this study was to develop a thermosensitive in situ gel (TISG) as an effective rectal delivery platform for delivering Periplaneta americana extracts (PA) to alleviate ulcerative colitis (UC) and explore the underlying molecular mechanism. 4Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou, China.3School of Pharmacy, Xuzhou Medical University, Xuzhou, China.2Department of Pediatrics, Xuzhou Medical University Affiliated Hospital, Xuzhou, China.1Institute of Pediatrics, Xuzhou Medical University, Xuzhou, China.Ming Wu 1,2, Hui Ding 1, Xiao Tang 3, Jiayi Chen 3, Meng Zhang 1, Ziqiong Yang 3, Qian Du 3,4* and Jun Wang 1,2*
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